Sysmex Norge
Sysmex Europe
Service
Support
Other Sites
Menu
  • GROWING YOUR KNOWLEDGE
    GROWING YOUR KNOWLEDGE
  • DEVELOPING YOUR SKILLS
    DEVELOPING YOUR SKILLS
  • HARVESTING EXPERTISE
    HARVESTING EXPERTISE

WBC differential channel

Analysing differentials consists of a cytochemical reaction of the cells with a reagent, followed by a fluorescence flow cytometric analysis.

A surfactant in the reagent induces the lysis of red blood cells. At the same time, cell membranes of white blood cells are perforated with ultramicroscopic pores through which a polymethine-based fluorescence marker migrates and binds to nucleic acids. This prepared sample is then analysed using fluorescence flow cytometry.

The measurement signals related to side scatter (SSC) and side fluorescence (SFL) are analysed and depicted in a scattergram.  Cells with similar cytochemical properties fall within the same area in the scattergram and can be separated using an advanced software algorithm.

The differential channel provides counts of all normal white blood cell subpopulations as well as flag information in cases of abnormalities.

 

 

RET channel

Fluorescence flow cytometry

DC sheath flow detection method

SLS detection
method

Our Glossary

An alphabetical listing of scientific terms

Explore our Glossary
Growing your knowledge
Visit our Academy
Grow your knowledge
Enjoy our Media Centre
All
  • All
  • Documents
  • Podcast
  • Images
  • Videos
Company movie
See our collection
See our collection
Grow your knowledge http://www.sysmex.no/nl-fr/academy.html
See our collection
See our library of smears http://www.sysmex.no/nl-fr/academy/library/images.html
Learn more about our concept http://www.sysmex.no/nl-fr/company/who-is-sysmex/silent-design.html